Blog 17 Comments on how to use NAD+, a possible step to the scientific fountain of youth, discovered at Harvard by David Sinclair, PhD. This Anti-aging article written by Mark Kingston Levin PhD an award-winning science fiction author and scientist.

Figure 1. David Sinclair PhD, Harvard Professor shown in the laboratory.

In 1906, the coenzyme NAD+ was first discovered by Arthur Harden and William John Young two British biochemists. Recently, the molecule was rediscover by David Sinclair and others for what it can do to restore and reverse aging.  Can it create a scientifically based Fountain of Youth for humans?

David A. Sinclair, PhD is best known for his work on understanding of the processes aging and how to slow them. He is a Professor in the Department of Genetics at Harvard Medical School but also maintains a lab at UNSW near Sydney Australia from where he came. He is co-Directors the Paul F. Glenn Center for the Biological Mechanisms of Aging. Dr. Sinclair obtained his PhD in Molecular Genetics at the University of New South Wales, Sydney in 1995. He then became as a postdoctoral researcher at MIT under the famous Dr. Leonard Guarente where he co-discovered a cause of aging for yeast as well as the role of Sir2 (The three letters Sir  stands for Silent Information Regulator, the 2 stand for the fact that the gene was the second SIR gene discovered and characterized. In 1999, Dr Sinclair was recruited to Harvard Medical School where his laboratory’s research has focused primarily on understanding the role of sirtuins in disease and aging, with associated interests in chromatin, metabolism, mitochondria, learning and memory, neurodegeneration, and cancer. He has been awarded more than 35 patents. Dr. Sinclair is co-founder of at least seven biotechnology companies including Sirtris, Genocea, Cohbar, MetroBiotech, ArcBio, Liberty Biosecurity and Ovascience. He has also received many awards and other recognition for his pioneering work. He is trying to find the scientific fountain of youth through his research, but we are not there yet. The future to watch is first human clinicals now underway. You can wait or jump in based on animal studies.

Figure 2. A new scientific Fountain of Youth giving us pills and supplements.

Figure 2. A new scientific Fountain of Youth giving us pills and supplements.

Figure 3 The art of getting younger. Comes from Harvard Magazine article about the anti-aging research of David Sinclair PhD.

Figure 3 The art of getting younger. Comes from Harvard Magazine article about the anti-aging research of David Sinclair PhD.

Strong evidence shows positive results in testing NAD+ in neurodegeneration in mice. However, a double-blind placebo-controlled clinical trial in humans failed. We must be cautious and careful. Double blind studies are underway, and we should wait, but I have started supplements of NR Branded as Niagen.

Toward the end of 2013, Dr. Sinclair discovered fame by working with colleagues to publish several papers on NAD effects on mice: these results shown the aging effect on mice reversing like from a FOUNTAIN OF YOUTH. While at MIT he opened-up the market for resveratrol, an ingredient in red wine. In the mid-2000s, David Sinclair and his colleagues first achieved fame for his work on an ingredient in red wine called resveratrol. This ingredient was expected to slow age in a similar way to calorie restriction functions.  It has not done anything for me that I can feel, yet.  I am not giving up. I do think this NAD+ is my best chance to reverse aging.

Figure 4. David Sinclair and some of his researcher team members near Sydney Australia. Professor David Sinclair (front) and Dr Lindsay Wu (far left) with the University of New South Wales research team. Photo: Britta Campion

Figure 4. David Sinclair and some of his researcher team members near Sydney Australia. Professor David Sinclair (front) and Dr Lindsay Wu (far left) with the University of New South Wales research team. Photo: Britta Campion

The NAD story took off in about 2013 with another high-profile paper by David Sinclair and his colleagues at Harvard. The headlines were picked-up around the globe. He reported that “the mitochondria in the muscles was restored to that of a youthful mouse after injection of This is a featured article.Nicotinamide adenine dinucleotide (NAD+) can added directly by injection as shown by David Sinclair OR it CAN BE STIMULATED BY CERTAIN PROMOTERS in the food such as nicotinamide mononucleotide(NMN) and another known as nicotinamide riboside (NR) both molecules occurring in normal cells and can be purchased on the internet as supplements. NAD+ as shown in Figure 5.

Figure 5. A model of the NAD+ molecule.

Figure 5. A model of the NAD+ molecule.

How can we medically restore our NAD+ when it is not stable molecule? We can inject into our bodies as done with the mice. There are also some other ways, for example, using supplements like NR or Niagen a band name. There are no proven methods in humans; however, there is hope as clinical human trials have started in August 2017. Please stay tuned as I expect first human efficacy trials to be completed soon but publication make take several additional months or years to see meaningful health effects and the impact on human lifespan.

Scientists speculate that NAD+ may contribute to longer life by repairing telomeres. I have not yet seen the data in humans; however, there is data in mice.  I am holding my breath.  I need this to work in humans for personal reasons.  I was diagnosed with multiple system atrophy (MSA), which is about the worst of the neurodegenerative disease.   I am cautious of course but looking for anything promising.

Research have shown that NAD+ is required for sirtuin proteins that contribute to longevity. How do they do this? One way is to maintaining the length of critical telomeres. Please see the two drawing below:

Figure 6. Shows chromosomes with telomeres located on the ends where the red cap is located all four ends. Telomeres are made up of DNA, Credits Bigstock.

Figure 6. Shows chromosomes with telomeres located on the ends where the red cap is located all four ends. Telomeres are made up of DNA, Credits Bigstock.

Figure 7 show what the telomeres are made from, which is DNA.

Figure 7 shows what the telomeres are made from, which is DNA.

Telomeres are repetitive pieces of DNA strands that are shown in yellow on the ends of chromosomes.  See the red and yellow caps area in the above figures 6 and 7 at the end of chromosomes. This is like a clock ticking to set off a bomb, our telomeres at the ends of our chromosomes gradually shorten every time a new cell is produced. Once telomeres reach a critically short length, death is near for that cell.

 

 

Researchers have been looking to find a drug and other interventions that might lengthen telomeres, to extend lifespan. To date, the best methods are weight loss and exercise. Certain other nutrients, such as resveratrol, may activate sirtuins. However, it has not worked for me, I tried drinking red wine and taking resveratrol. After many years no effect.

 

NAD+ is unstable and cannot be used as a pill supplement. Humans prefer taking a pill to injections. Nicotinamide riboside (NR) is a useful precursor to NAD+. Data shows that NR is capable of restoring cellular NAD+ levels in mice and recently in humans.

Animal studies show that nicotinamide riboside supplementation can slow cellular aging and improve many of the metabolic defects common to the aging processes in mice, including obesity, cardiovascular disease, neurodegenerative conditions and more.

Supplementation with nicotinamide riboside offers a way of supporting essential body systems with NAD+ in humans, but health effects, which take years to study, must be completed to really know the benefits of this potential fountain of youth. I am waiting for the publications. But I am taking NR the precursor and think I feel something called more stamina.  It is only subjective. We all need real double-blind placebo-controlled data.

With recent research coming from David Sinclair’s (the Australian transplant) lab at Harvard shows that in advanced age, the cell NAD+ levels plummet. Theodore, restoring normal NAD+ levels in mice showed dramatic improvements. David Sinclair once implied that normal aging may one day be classified as “NAD+ deficiency syndrome.”

Recent research suggests it may be possible to reverse mitochondrial decay with dietary supplements that increase cellular levels of a molecule called NAD (nicotinamide adenine dinucleotide) in animals, so let us hope they work on all humans.  I beat two incurable and untreatable LGL leukemias (t cell and nk) after about ten-year battle with the help of Dr. Catriona Jamieson at UCSD and a new drug recently developed by J&J called Imbruvica.  I contracted Wolhdenstrom’s Syndrome, which is a b-cell lymphoma in about 2012-2013.  I tried all chemo and other drugs available nothing worked until Imbruvica was approved on Dec. 19, 2014. Dr. Jamieson started treatment with Imbruvica in early January 2015 and by April 2017; Dr. Jamieson told me I was no longer her patient because she cannot find any cancer in my body!!  I had many failures and one success. I am now ready to fight against Multiple System Atrophy (MSA). Dose is one gram per day has been shown in small studies to increase NAD levels in humans (Charles Brenner and the University of Boulder study). The NAD structure is shown below. This molecule may be the scientific fountain of Youth, stay tuned for more proof as it become available.

Figure 8. Structure of nicotinamide adenine dinucleotide, oxidized (NAD+). (Image: NEUROtiker/Wikimedia)

Figure 8. Structure of nicotinamide adenine dinucleotide, oxidized (NAD+). (Image: NEUROtiker/Wikimedia)

Dr. Levin

Dr. Levin was born and grew up in Vermont with many winters spent in Florida as a child. As a teenager he wrote poetry, served as a lifeguard and played football. He currently enjoys sailing, exploring underwater caves, snorkeling, writing science fiction and other pursuits. After working on the Apollo and Mars projects, he returned to school to study under Nobel Laureate Paul Dirac, obtaining his PhD in 2.5 years. Dr. Levin founded two companies and served the science policy apparatus in President Ford’s administration. He has been published over 44 times in scientific literature and was awarded over 32 US patents. The science fiction writer is now emerging with his first work, a trilogy entitled 30th Century. The first award-winning book, 30th Century: Escape, is currently available on Amazon. Book two in the series, 30th Century: Revived, should be released before the end of April 2018.

2018-12-10T22:06:27+00:00 May 8th, 2018|Blog, Human physiology|